mtt cell viability kit  (Biotium)

Journal: Journal of Clinical Medicine

Article Title: Characterization of the Effects of a Humanin Fragment Peptide (HNF 14 ) in Age-Related Macular Degeneration

doi: 10.3390/jcm15051686

Figure Lengend Snippet: Effects of HNF 14 on cellular metabolic activity in AMD cybrids. Cellular metabolic activity was assessed using an MTT assay in normal and age-related macular degeneration (AMD) transmitochondrial cybrid cells treated with HNF 14 (3.2 µM) for 48 h. Individual data points represent biological replicates, and horizontal bars indicate the mean ± SD ( n = 5 biological replicates). Data were normalized to untreated normal cybrids. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.

Article Snippet: AMD and normal cybrids plated in 96-well tissue culture plates were treated with MTT solution (Catalog number: 30006, Biotium, Fremont, CA, USA) at the 48 h time point.

Techniques: Activity Assay, MTT Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: The Role of HER2 and RANK in Breast Cancer and New Therapeutic Approaches With Denosumab, Anti‐HER2 Antibodies and Immunotherapy

doi: 10.1111/jcmm.71071

Figure Lengend Snippet: Cell viability levels of SK‐BR‐3 and MCF‐7 determined by MTT assay. (A, B) Effects of single (A) and in combination (B) therapeutic agents on cell viability in HER2 + (SK‐BR‐3) and HER2 − (MCF‐7) breast cancer (BC) cell lines. The data is expressed as mean ± SD of three independent experiments ( N = 3). Statistical significance was determined by ANOVA followed by Dunnett's T3 post hoc test, where * p ‐value < 0.05; ** p ‐value < 0.01; *** p ‐value < 0.001 in comparison with the control group. (C) Experiments at the level of 3D cell lines, when incubated with D+T+P+N+I (Denosumab+Trastuzumab+Pertuzumab+Nivolumab+Ipilimumab) in HER2 + BC, resulted in a clinically significant reduction in cell viability, possibly through immunomodulation of the tumour microenvironment (TME), characterised by a marked increase in tumour‐infiltrating lymphocytes (TILs) infiltration and a possibly concurrent decrease in pro‐tumorigenic neutrophils (created using BioRender ( https://biorender.com/ )). ANOVA, analysis of variance; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; SD, standard deviation.

Article Snippet: Following treatment, the reagent 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) (30006, Biotium Inc., Landing Parkway, Fremont, CA, USA) was added to each well at a final concentration of 0.5 mg/mL.

Techniques: MTT Assay, Comparison, Control, Incubation, Standard Deviation